Ethyl Acetate Fraction Of Secang As Anti Cervical Cancer By Inducing p53 and Caspase 9

Secang (Caesalpinia sappan L.) contains potentially anti-cancer active compounds, namely brazilin and brazilein which are soluble in ethyl acetate. This study is conducted to evaluate the anticancer activity of secang wood in cervical cancer cells (HeLa) in vitro and molecular mechanisms that selectively mediate their cytotoxic effects on several cancer cells through molecular docking or in silico. The anticancer potential of the ethyl acetate fraction of secang wood through its cytotoxic activity on HeLa cells was carried out with MTT assay and calculated the IC50 value. The molecular mechanism is carried out in silico with the target protein p53 and caspase 9 using the autodock 4.2 program. The docking process includes protein and active compounds preparation, validation of molecular docking methods and docking brazilein as the active substance with the target protein. The docking score is measured by binding energy value between brazilein and the target protein. The fraction of secang wood was cytotoxic in HeLa cells with IC50 values of 48.71 ?g/mL. The docking results showed that brazilein had an affinity for the target proteins p53 and caspase 9 with binding energies of -8.24 and -6.71 kcal / mol, respectively.