Secretion of Geobacillus thermoleovorans IT-08 Alpha-L-Arabinofuranosidase (AbfA) in Saccharomyces cerevisiae by Fusion with HM-1 Signal Peptide

Two different expression vectors were constructed to investigate two signal peptides on secretion of Geobacillus thermoleovorans IT-08 alpha-L-arabinofuranosidase (AbfA) in Saccharomyces cerevisiae. They were designed to direct the secretion of AbfA by the aid of one of the following signal peptides, the alphaF signal peptide in the plasmid YEpFLAG1-Af and HM-1 signal peptide in the plasmid pYHM1-Af. Although some successful results have been reported in proteins secretion with alphaF leader sequence, in this research no alphaL-arabinofuranosidase activity could be observed in recombinants S. cerevisiae YEpFLAG1-Af. The HM-1 leader sequence, originated from Hansenula mrakii IFO 0895 killer toxin, showed the capability to AbfA secretion.