The Response to Oxidative Stress Alpha-humulene Compounds Hibiscus manihot L Leaf on the Activity of 8-Hydroxy-2-Deoksiquanosin Levels Pancreatic Beta-Cells in Diabetic Rats

ABSTRACT Increased antioxidant activity of ?-humulene of Hibiscus manihot L leaf empirically may help prevent oxidative stress due to the production of compounds reactive oxygen species (ROS), thereby protecting cells from damage function. Oxidative stress can be formed through an increase in the activity of the compound 8-hydroxy-2-deoksiguanosin in the body. This study is a laboratory experimental design was randomized pre and post-test control group design. A total of 36 wistar rats were divided into 4 groups, namely P 0(negative control), group P 1 (isolate ?-humulene dose of 5 mg/kg, P 2 (isolate ?-humulene dose of 10 mg/kg), and P 3 (isolate ?-humlene dose of 15 mg/kg ). All groups induced by alloxan dose of 140 mg/kg in order to get the condition. After the treatment for 12 weeks, serum was taken for examination of 8-OHdG levels and see a picture immunohistochemistry pancreatic tissue of wistar rats. Data were analyzed by one-way ANOVAand continued test t-pairedto determine the mean difference between treatments with significance limit of 5%. the results showed that, the dose ?-humulene isolate of Hibiscus manihot L resulted in a decrease in the average of 8-hydroxy-2-deoksiguanosin levels in the positive control group was (3.63 ± 0, 19) ng / mL, the treatment groups respectively P 1 by (3.94 ± 0.51) ng/ mL; P2was (3.49 ± 0.47) ng/mL and P3 of (1.99 ± 0,18) ng/mL. It is also evident from the results of immunohistochemical against 8-OHdG profile repair due to damage pancreatic ?-cells in the islets of Langerhans. This difference was statistically significant was shown with a value of p<0.05. Therefore ?-humulene compounds Hibiscus manihot L leaf may help prevent oxidative stress through reduction mechanism 8-OHdG Keywords: Oxidative Stress, ?-humulene, Hibiscus manihot L, 8-OHdG, Diabetes