Antioxidant Compounds of Gayam Seed (Inocarpus Fagiferus Fosb) to Prevent Atherosclerosis in Wistar Rat With High Cholesterol Diet

Gayam seed (Inocarpus fagiferus Fosb) is empirically has an ability to reduce oxidative stress diseases such as atherosclerosis. This study aims to prove the antioxidant compounds such as linoleic acid, ethyl linoleic, ethyl oleic, and homopterocarpine in ethanol extract of gayam seed (Inocarpus fagiferus Fosb) to prevent atherosclerosis through increase SOD-3 expression and decrease of MDA concentration plasma blood of Wistar rat with high cholesterol diet for 16 weeks. This was an experimental study with randomized posttest only control group design. The samples were 25 Wistar rat, randomized into 5 treatment groups, i.e. group P0 (negative control), group P1 (positive control, feed high cholesterol diet), group P2 (high cholesterol diet and ethanol extract in dose of 50 mg/kg bw), group P3 (high cholesterol diet and ethanol extract in dose of 100 mg/kg bw), and group P4 (high cholesterol diet and ethanol extract in dose of 150 mg/kg bw). After 16 weeks treatment, blood of the rats were driven for MDA and all rats were then euthanasia to obtain their aorta for immunohistochemistry analyzed to give expression of SOD-3 data. All of data was analyzed by Anova to obtain the treatment different toward control by statistically with significance at a =0.05. The results showed that ethanol extracts of gayam seed (Inocarpus fagiferus Fosb) in doses of 50-150 mg/kg bw increase the expression of positive SOD-3 aorta endhotel cell and decrease the concentration of MDA significantly (p<0.05). The expression of positive SOD-3 aorta endhotel cell is marker endogenous antioxidant, therefore, the antioxidantcompounds in ethanol extract of gayam seed can to prevent atherosclerosis disease through of SOD inducer mechanism.