Basic Study Microspore Development of Indonesian sugarcane (Saccharum officinarum L.)

There is no standart protocol for microspore culture sugarcane. This reseach showed the first method to determine microspore development for preparation microspore culture sugarcane. This study used BL variety. The Stage of microspore was checked under microscope. All the experiment was designed according to CRD (completely random design) and the data was analyzed using descriptions technique. the result is each anther color has a different percentage of stages of microspores. Besides that from three sampling location that is jember, lumajang and bondowoso, percentage of number of microspore stages obtained also vary. The highest percentage of uninucleate stage was found in the yellow anter of 94.27% from Jember sampling area. Keywords: saccharum, embryogenesis, uninucleate stage