Topical Anti-inflamatory Activity Test and Identification of Anti-inflamatory Active Compounds of Ether Extract of Stem Bark and Leaves Resin of Tenggulun Protium javanicum Burm

ABSTRACT This research aimed to evaluate the topical anti-inflammatory activity of the ether extract of stem bark and resin of tenggulun leaves on the ears inflamed rats that were induced by croton oil. The ether extract of the stem bark and resin of tenggulun leaves was purified by gradient elution column chromatography. The extract resulted by the column chromatography was identified for the compound components by GCMS and then the test for the topical anti-inflammatory activity on the ears inflamed rats was carried out. The GCMS result suggested that there were some secondary metabolites as follows: the dichloromethane fraction of the stem bark extract contained stigmasterol, lanosterol, cholesterol, ergosterol, picenon, lupeol and ?-amyrin. The ethyl acetate fraction contained stigmasterol, lanosterol, cholesterol, ergosterol, lupeol, neogamaseron and stigmastenon. The dichloromethane fraction in the leaves resin contained limonene (61.74%), caryophyllene (23.07%), naphthalene (2.62%), isolongifolol (1.22%), spathulenol (11.35%). The ethyl acetate fraction contained limonene (13.09%), caryophyllene (14.85%), spathulenol (17.26%), lanosterol (31.50%) and epiglobulol (23.30%). The diethyl ether fraction contained caryophyllene (17.50%), spathulenol (28.67%), epiglobulol (18.51%), naphthalenol (0.69%), azulena (0.55%), bisabolen (6.19%), androstenon (6.19%) and dodecanediol (21.70%). The result of topical of anti-inflammatory activity test indicated that the stem bark and resin of tenggulun leaves could be used as an anti-inflammatory. The capability in inhibiting inflammation was more than 50%. Key words: topical anti-inflammatory, ether extract, Protium javanicum burm, gradient elution, column chromatography