Approach of Aerob-Anaerob Biosystem Treatment On Biodegradation Of Remazol Brilliant Blue.

The research about Remazol brilliant blue (RBB) wastewater treatment was conducted due to several issues related with dye pollutions in some rivers which surrounded by immersion and garment industries. The active suspension of microorganism inoculation into biosystem need to be advanced to enhance the effectivity of dye wastewater treatment. The soil resources which used to plant microorganisms take an important role in defining the ability of active suspension. This research was intended to obtain the finest active suspension that planted from several rivers sediment, moreover, to determine the effectivity and treatment capacity of vertical flow biosystem. The specific method was applied to specify the best active suspension by planting soil samples (purposive) inside selective media which contained RBB substances. Biomass growth was calculated by determining the Mixed Liquor Volatile Suspended Solid (MLVSS), and then the finest suspension was acquired from the fastest biomass growth. Furthermore, the finest active suspension was inoculated into vertical plant biosystem. The consortium of microorganisms in active suspension was built up in form of biofilm by immobilize it into volcanic rocks (biosystem) during 7 days before applied to treat RBB wastewater. Thus, the effectivity and treatment capacity of the biosystem was defined by calculating the concentration of RBB artificial wastewater from vertical biosystem and identified the bacteria species which involved in this treatment. The result showed that the best active suspension was obtained from soil sediment of river around the industries, the highest Mixed Liquor Volatile Suspended Solid (MLVSS) concentration was 1750 mg/L during 48 hour breeding time. In the biosystem, RBB removal occurred in the 6-hours treatment to the 114-hours, from 200 mg/L decline into 19.6211 mg/L. Then, it rose again into 19.8209 mg/L in the 120-hours. It figured the removal efficiency about 90.19 % in 114 hours treatment, whereas the treatment capacity was 1.6525 x 10-4 mg/gram along 114 hours. The specific bacteria which took the main role were Pseudomonas sp., Aeromonas sp, and Plesiomonas sp. However, Pseudomonas sp. was the most prominent one who generated the tremendous initial colony amount of 7.2 x 104 CFU/gram before degradation process and 2.6 x 103 CFU/gram after degradation. Keywords: Biodegradation, active suspension, Remazol Brilliant Blue, Inoculum, Vertical Biosystem