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Journal article
Total Phenolic and Flavonoid Contents and Antimicrobial activity of Acorus calamus L. Rhizome Ethanol Extract
Wiwik Susanah Rita RETNO KAWURI I Made Dira Swantara
Volume : 22 Nomor : 2 Published : 2018, August
Research Journal of Chemistry and Environment
Abstrak
The rhizomes of Acorus calamus L. are widely used as medicinal plant for diseases caused by microbes. The antimicrobial activity of rhizomes is suspected to have a positive correlation to the content of phenolic and flavonoid compounds. This study aims to determine the total phenolic and flavonoid contents and to evaluate the antimicrobial activity of ethanol extract of Acorus calamus L. rhizome. An extraction process was carried out at room temperature by maceration methods. The determination of total phenolic and flavonoid contents was conducted by a UV-Vis Spectrofotometer using standard gallic acid and quercetin respectively. Additionally, the antimicrobial activity was evaluated by the agar disc diffusion method. The total phenolic and flavonoid contents were successively 2398.40 mg GAE/100g and 190.46 mg QE/100g dry rhizome extract. The extracts demonstrated antibacterial activity against Escherichia coli and Staphylococcus aureus and antifungal activity against Candida albicans. Minimum inhibitory concentration of the extracts against Escherichia coli, Staphylococcus aureus and Candida albicans was 2, 3 and 3% respectively with the inhibition zone of 9.80, 9.50 and 8.67 mm. Keywords: Acorus calamus L., Antimicobial Activity, Flavonoid contents, Phenolic contents.