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Journal article
Optimization of the Production of Microbial Cellulose by Acetobacter xylinum in Aloe barbadensis Mill. Medium
derik RETNO KAWURI Yan Ramona Gary Cass
Volume : 2 Nomor : 6 Published : 2014, December
International journal pure and apllied bioscience
Abstrak
Aloe barbadensis Mill. was used as an alternative medium for growing A. xylinum culture to produce Microbial Cellulose (MC). A one year and a six months old A. barbadensis Mill. leafs were mixed to produce A. barbadensis Mill.-medium (ABM-medium). The mixture ratio of the one year and six months old Aloe barbadensis Mill. are 100%:0%; 75%:25%; 50%:50%; 25%:75%; 0%:100% respectively. Distilled water was used as control. These media were then fermented for seven days in 28 C. The dry and wet weight data of the produced MC were collected and analyzed using one way-ANOVA. The result showed that ABM-media produced more MC than the control (P ? 0,05) and the optimum ratio of the ABM-medium is 0% old:100% young based on the wet and dry weight. This result was then used in the second experiment. Initial pH of 3, 5, 7 and 9 were combined with glucose concentration of 0% (control); 2,5%; 5%; 7,5%; 10%; 12,5% and 15% using factorial ANOVA design to get the optimum combination of both in the ABM-medium. After seven days of fermentation in 28 0C the result showed that the optimum combination of all is pH 3 with 15% glucose (P ? 0.05) with the highest wet and dry weight of MC. Keywords: Acetobacter xylinum, Microbial cellulose, Aloe barbadensis Mill., ratio, medium.