Antibiosis Profile Compound of Trichoderma sp. in Attempt of Controlling Aspergillus flavus in Balinese Lontar

ABSTRACT Lontar is an important inheritance to be preserved. The writing in the Lontar contains an ancient Balinese script. Poor environmental conditions can cause the Lontar to be damaged. One of the cause of the damage is Aspergillus flavus fungi which is able to produce selulase enzyme. The enzyme may damage the basic component of the Lontar. An attempt to achieve an antibiosis having a fungicidal feature is needed to be done, which is by using Trichoderma. Trichoderma is a group of fungi that has the ability to inhibit the growth of other organism. Based on this fact, therefore a research was done in order to understand the antibiosis compound profile produced by Trichoderma sp. in controlling A. flavus in Balinese lontar. The isolation of A. flavus and Trichoderma sp. was done by using the plating method in sequential dilution. The identification of the Trichoderma sp. was done conventionally according to the book of identification by Pit and Hocking (1997). To test the inhibition strenght of Trichoderma sp. against the A.flavus, the dual culture methode was used, while the diffusion well methode was used to find test the inhibitory strenght of the Trichoderma sp. filtrate extract against the tested fungi. Antibiosis compound profile of the Trichoderma sp. was known by using the colomn dan GCMS (Gas Chromatography Mass Spectrometry) technic. The in vivo test of the inhibitory strenght of the Trichoderma sp. filtrate extract against the A.flavus was designed by using 6 treatment and 4 repetition. The result of the study shows the identified A. flavus isolated from Balinese Lontar and Trichoderma sp. identified from isolation result of rhizosphere of pea plant Trichoderma sp. has the strenght to inhibit the A. flavus in the percentage of 69,5±1,56 % and the obtained filtrate extract can form inhibitory zone in the percentage of 15,32±0,277 mm. The 15 compound identified in filtrate extract is allegedly act as an antibiosis agent namely Benzene,1,3,5-trimethyl-(CAS) 1,3,5-trimethyl; 2-Azabicylol(3,2,1)octan-3-one; 5,6-Dihydro-4-methyl-2H-Pyran-2-C; 2-Butenoic acid, 2-methyl-(CAS)2-methyl-2; 2H-Pyran-2-one,tetrahydro-4-hydroxy-4-methyl; 2-Bromo dodecane; Lyxitol,1-O-nonyl; Pentadecane,3-methyl-(CAS)3-methylpentax; Trichloroacetic acid, tridecyl ester; Heptadecane,3-methyl-(CAS)3-methylhepta; n-Hexadecanoic acid; Nonadecane,3-methyl-(CAS) 3-Methylnonad; Octadecanoic acid; 3-Methylheneiccosane and Heptadecane,3-methyl. The filtrate extract of the Trichoderma sp. with the concentration of 1,56/20?L based on the in vivo test (Balinese Lontar) shows a significant different (P?0,05) in compare with the treatment without Trichoderma sp. filtrate extract. Key word : Antibiosis, Trichoderma harzianum, Aspergillus flavus , Ekstrak and Filtrat. J.