EN 
IN
Journal article
Antibacterial effect of Nocardia sp against Methicillin Ressistent Staphylococcus aureus MRSA
Raningsih RETNO KAWURI Ida Bagus Gede Darmayasa
Volume : 3 Nomor : 4 Published : 2015, August
International Journal of Pure & Applied Bioscience
Abstrak
Pathogenic bacterial resistance against antibiotic was one of major problem in medical concern. Staphylococcus aureus or Methicillin Resistant Staphylococcus aureus (MRSA) is one of those pathogenic bacteria. This research purposes to discover the new antibacterial substance using strain indentified as Nocardia sp. which belongs to Actinomycetes. Bacterial isolate was collected from Udayana University Microbiology Laboratory, Indonesia. Antagonistic evaluation using pour plate method in Nutrient Agar (NA) medium shows Nocardia sp. 1 strain has the highest result of inhibition zone test (22.7 mm long) against MRSA compared to 6 other strain. Nocardia sp. 1 strain filtrate tested on MRSA has a MIC level of 2%. Later identification of chemicals substance contained in Nocardia sp. 1 strain filtrate detects substances such as 3,5 -Dichloro- 2 pyridone, Benzeneacetic acid, Hepatdecane, Phenol. 2.4-bis(1.1-dimethylethyl), Dodecanoic Acid Methyl Ester, Hexadecanoic Acid Methyl Ester, 1-(+)-Ascorbic Acid 2.6 Dihexadecanoate. Key words: Antibacterial, Actinomycetes, Methicillin Resistant Staphylococcus aureus, MIC.