Antifungal Activity of Ficus septica Leaf Extract against Colletotrichum gloeosporioides Cause Anthracnose Disease on Local Fruits in Bali

A research on antifungal activity assay leaf extract of awar-awar (Ficus septica Brum .f) against Colletotrichum gloeosporioides causes anthracnose disease on local fruits in Bali has been conducted. The study was conducted at the Laboratory of Biochemistry Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Udayana Bukit Jimbaran with the extraction method and diffusion wells. The study was designed with a completely randomized design (CRD) comprised of 5 treatments with 5 replications. Qualitative data were analyzed by descriptive and quantitative data were analyzed by analysis of variance (ANOVA), when the data show significant differences continued with DMRT at 5% level. The results showed that the leaf extract of awar-awar can inhibit the growth of Colletotrichum gloeosporioides with the formation of inhibition zone around the well diffusion. Colony growth, formation of spore, germination of spores and fungal biomass C. gloeosporioides can be inhibited by a leaf extract of awar-awar significantly (P<0, 05) when compared with controls.