Journal article

SCREENING AND IDENTIFICATION OF SUPERIOR LIGNOCELLULOSE DEGRADING BACTERIA FROM TERMITES

I Made Mudita I Gede Mahardika Ida Bagus Gaga Partama I NENGAH SUJAYA Ni Nyoman Suryani I Wayan Suarna

Volume : 0 Nomor : 1 Published : 2019, January

Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and Chemical Sciences

Abstrak

A research had been carried out to screening and identification of superior lignocellulose degrading bacteria isolates from termites. Screening of lignocellulose bacteria degrader based on lignocellulase specific enzyme activities such as ligninase, cellulase (endoglucanase and exoglucanase) and xylanase. Identification of superior bacteria isolates carried out by molecular biology technologies (PCR).The result showed that from termites has isolation 36isolates of lignocellulosic degrading bacteria such as 10 lignocellulolytic bacteria, 7 lignolytic bacteria, 9 cellulolytic bacteria and 10 xylanolytic bacteria isolates. Based on lignocellulase specific enzyme activities, thesuperior lignocellulolytic bacteria is Aneurinibacillus sp. strain BT4LS (homology 99% with Acc. No. KR063553); superior lignolytic bacteria is “Aneurinibacillus sp. strain BT5LG” (homology 81% with Acc. No. KP980744); the superior cellulolytic bacteria is “Bacillus sp. strain BT3CL” (homology 100% with Acc. No. KX879851), and the xylanolytic bacteria “Bacillus sp. strain BT8XY” (homology 100%with Acc. No. KT981879).