POTENCY LIGNOCELLULOSE DEGRADING BACTERIA ISOLATED FROM BALI CATTLE RUMEN CONTENT WASTE AND TERMITES AS NONCONVENTIONAL WASTE DEGRADER

A research has been carried out to evaluating potency lignocellulose degrading bacteria isolated from bali cattle rumen content waste and termites. Those animals were chosen because they were has consume low quality crude fiber as their main energy sources. Lignocellulose degrading bacteria were isolated by Hungate selective media, using lignin (tannic acid), xylan, and cellulose as selective substrates. The best lignocellulose degrading bacteria then was determined by enzyme activity by isolate. This research showed that lignocellulose degrading bacteria could be found in bali cattle rumen content waste and termites. In bali cattle rumen content waste found 4 isolate lignocellulolytic, 5 isolate cellulolytic, and 5 isolate xilanolytic bacteria. Even though from termites found 8 isolate cellulolytic and 1 isolate xilanolytic. Enzyme activity evaluation showed first and second highest lignocellulolytic, cellulolytic, and xylanolytic activities from bali cattle rumen content waste isolates were reached by BCR Ligsll 2 and isolate BCR Ligsll 4, isolate BCR CMC 2-2 and isolate BCR CMC 2-3, BCR Xy 2 and BCR Xy 3. Meanwhile from termites first and second highest cellulolytic, and xylanolytic activities were reached by BR CMC 3-7 and BR CMC 3-2, isolate BR Xy. It was concluded there nine isolate bacteria has highest enzyme activity were chosen as nonconventional waste degrader for bali cattle feed production.