POPULASI BAKTERI PELARUT FOSFAT DAN KARAKTERISTIK BERBAGAI JENIS MEDIA TANAM DAN PUPUK ORGANIK

POPULASI BAKTERI PELARUT FOSFAT DAN KARAKTERISTIK BERBAGAI JENIS MEDIA TANAM DAN PUPUK ORGANIK Putri. S. S., I. K. M. Budiasa, dan N. G. K. Roni PS Sarjana Peternakan, Fakultas Peternakan, Universitas Udayana, Denpasar, Bali e-mail: shofia25putri@gmail.comTelp:085692348113 ABSTRAK Bakteri pelarut fosfat (BPF) merupakan salah satu jenis mikroba tanah yang berperan penting dalam membantu penyediaan unsur hara dalam tanah terutama fosfor (P). Penelitian ini bertujuan untuk mendapatkan data total plate count (TPC), populasi bakteri pelarut fosfat (BPF), derajat keasaman (pH) dan suhu pada berbagai jenis media tanam dan pupuk organik. Rancangan yang digunakan adalah rancangan acak lengkap (RAL), terdiri atas tujuh perlakuan yaitu: tanah (M1), pupuk kotoran sapi (M2), slurry (M3), bio-slurry (M4), tanah yang dipupuk kotoran sapi (M5), tanah yang dipupuk slurry (M6), dan tanah yang dipupuk bio-slurry (M7). Setiap perlakuan diulang tiga kali sehingga terdapat 21 unit percobaan. Variabel yang diamati dalam penelitian ini yaitu total plate count (TPC), bakteri pelarut fosfat (BPF), derajat keasaman (pH), dan suhu. Data yang diperoleh dari penelitian ini dianalisis menggunakan analisis sidik ragam, apabila terdapat perbedaan yang nyata (P<0,05) maka analisis dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa TPC diperoleh hasil berkisar 9,40 × 107 – 1,84 × 109 cfu/g, BPF diperoleh hasil berkisar. 7,23 × 106 – 1,77 × 108 cfu/g. Derajat keasaman (pH) tertinggi pada perlakuan M5 sebesar 7,00. Suhu tertiggi pada perlakuan M3 dan M4 sebesar 31,00oC. Berdasarkan hasil penelitian dapat disimpulkan bahwa populasi total plate count (TPC) dan populasi bakteri pelarut fosfat (BPF) paling banyak pada pupuk organik bio-slurry, derajat keasaman (pH) meningkat pada media tanam pupuk kotoran sapi dan pupuk organik slurry dan bio-slurry, sedangkan suhu meningkat pada perlakuan pupuk organik kotoran sapi, slurry, dan bio-slurry. Kata kunci: Bakteri pelarut fosfat, pupuk kotoran sapi, slurry, bio-slurry POPULATION OF PHOSPHATE SOLUBILIZING BACTERIA AND CHARACTERISTICS IN VARIOUS TYPES OF GROWING MEDIA AND ORGANIC FERTILIZER ABSTRACT Phosphate solubilizing bacteria (BPF) is one type of soil microbes that plays an important role in helping supply nutrients in soil, especially phosphorus (P). This study aims to obtain total plate count (TPC) data, population of phosphate solubilizing bacteria, characteristics of acidity Submitted Date: Oktober 15, 2019 Accepted Date: October 20, 2019 Editor-Reviewer Article;: A.A.Pt. Putra Wibawa & I Wyn Wirawan Putri, S. S.. et al, Peternakan Tropika Vol. 7 No. 3 Th. 2019: 1082 – 1095 Page 1083 (pH) and temperature on various types of planting media and organic fertilizers. The design used was a completely randomized design consisting of seven treatments, namely: soil (M1), cow manure (M2) slurry (M3), bio-slurry (M4), soil fertilized with cow manure (M5), soil fertilized with slurry (M6), soil fertilized with bio-slurry (M7). The treatments were repeated three times so that there were 21 experimental units. The variables observed in this study were total plate count (TPC), phosphate solubilizing bacteria (BPF), acidity (pH), and temperature. The data obtained from this study were using analyzed variance analysis, if there were significant differences (P <0.05) then the analysis continued with Duncan's multiple range test. The results showed that the TPC obtained results ranged from 9.40 × 107 - 1.84 × 109 cfu / g, BPF obtained results ranged 7.23 × 106 - 1.77 × 108 cfu / g. The highest of acidity (pH) in the M5 treatment is 7.00. The highest temperature in the treatment of M3 and M4 is 31,000C. Based on the results of the study concluded that the most population of the total plate count (TPC) and population of phosphate solubilizing bacteria (BPF) at bio-slurry organic fertilizer. The acidity (pH) was increased in cow manure and slurry and bio-slurry organic fertilizer media, while the temperature increased in the treatment of cow manure, slurry, and bio-slurry organic fertilizer. Key words: phosphate solubilizing bacteria, cow manure, slurry, bio-slurry