Degradation of Lignocellulosic Subtrates by Bacteria Isolated from Waste Landfill

A research has been carried out to evaluating potency of bacteria isolated from waste of general rubbish place landfill as lignocellulosic substrates degrader. Bacteria were isolated by Hungate selective media using combination of tannic acid (lignin), xylan, and Carboxy Methyl Cellulose/CMC (cellulose) as selective substrates. The potency of lignocellulolytic was identified based on degradation of lignocellulosic substrates measurement using clear/diffusion zone wide and lignocellulase enzyme activity. This study showed that lignocellulolytic bacteria isolated from waste landfill produce clear/diffusion zone wide 0.150 – 3.603 cm2 , 0.010 – 0.051 cm2 , 1.343 – 1.565 cm2 and 0.260 – 0.435 cm2 respectively for lignocellulose, lignin, cellulose and xylan substrates, while isolates coded BW 1 LC produced highest clear/diffusion zone wide on lignocellulose, lignin and xylan substrates, even though on cellulose substrates, bacteria isolates coded BW 4 LC highest clear zone wide. Meanwhile for lignocellulase enzym activity, bacteria isolates coded BW 1 LC and BW 4 LC has highest enzyme activity at tannic acid, CMC, and xylan substrates. It was concluded that bacteria isolates coded BW1 LC and BW 4 LC were potential as lignocellulosic substrates degrader. Keywords – Waste Landfill, Clear Zone Wide, Enzyme Activity, Lignocellulosic Substrate