Isolation of saccharomyces spp. from manure of beef Bali cattle as a probiotics properties and has CMC-ase activity to improve nutrient quality of rice brand

ISOLATION OF Saccharomyces spp FROM MANURE OF BEEF CATTLE AS A PROBIOTICS PROPERTIES AND HAS CMC-ASE ACTIVITY TO IMPROVE NUTRIENT QUALITY OF RICE BRAN DESAK PUTU MAS ARI CANDRAWATI, D.A. WARMADEWI, AND IGNG. BIDURA Faculty of Animal husbandry, Udayana University, Denpasar-Bali Jl. PB. Soedirman, Denpasar. Phone/fax. 0361-702771; Email: dsk_candrawati@yahoo.co.id ABSTRACT ABSTRACT: This experiment was carried out to study both on the CMC-ase activity and probiotics agent of Saccharomyces spp isolation from feses of beef cattle samples can be used in order to alleviate the negative effect of rice bran as feed. Eighteen of Lohman Brown laying hens was assigned to three treatments in a completely randomized design. Each treatment has six replications with one birds per replication (individual cage). The treatments were (i) unfermented rice bran as control; (ii) fermented rice bran by Saccharomyces spp.S-6 isolate; (iii) fermented rice bran by Saccharomyces spp.S-7 isolatse, respectively. The report on the first experiment showed that there are five isolate of Saccharomyces spp (Sc.S-5; S-6; S-7; S-8; and S-9) were isolated from manure of beef Bali cattle samples in the first experiment. All of isolate Saccharomuces spp showed resistant grew on both in different temperature (100-450C), acid conditions (2,5-4,5), and bile salt. But only two isolate (Sc.S-6 and S-7) both were the potensial as a probiotics sources and has CMC-ase akctivity. The study showed that fermentation of rice bran using both of Saccharomyces spp.S-6 and S-7 culture could improve significant differences (P<0.05) on digestability of its dry matter (DM), organic matter (OM), crude protein (CP), crude fibre (CF), and increased its metabolizable energy of rice bran. The content of dry matter, organic matter, and gross energy of rice bran were no effected significantly different (P>0.05) by fermentation. On the other hand fermentation caused increasing crude protein (CP) and crude fibre (CF) of the rice bran. It was concluded that there are two isolate (Sc.S-6 and S-7) were isolated from manure of beef Bali cattle samples, both were the potensial as a probiotics sources and has CMC-ase akctivity and its utilization in the rice bran fermentation could increase nutrient composition and digestibility of rice bran. Key words: Saccharomyces spp., probiotics, crude fiber, digestibility, rice bran