Implementation of Saccharomyces Spp.S-7 Isolate (Isolated From Manure of Bali Cattle) as A Probiotics Agent in Diets on Performance, Blood Serum Cholesterol, and Ammonia-N Concentration of Broiler Excreta

Abstract: The study was carried out to study the effect of supplementation of Saccharomyces spp.S-7 isolate (isolated from manure of Bali cattle) in basal diets on performance, breast meat, abdominal-fat, blood serum cholesterol, and concentration N-NH3 of broiler excreta. One hundried twenty of male broiler aged two of weeks was assigned to four treatments in a completely randomized design. Each treatment has six replications with five birds per replication. All of the birds were fed experimental diets for four weeks. The treatments were (i) diets without supplemented of Saccharomyces spp.S-7 isolate culture as control; (ii) supplemented of 0.20% Saccharomyces spp.S-7 isolate in diets; (iii) supplemented of 0.40% Saccharomyces spp.S-7 isolate in diets; and (iv) supplemented of 0.60% Saccharomyces spp.S-7 isolate in diets, respectively. The study showed that supplementation of Saccharomyces spp.S-7 isolate culture in diets (treatment B, C, and D) could improve significant differences (P<0.05) on performance of broiler. Carcass percentage and breast meat of birds treatment B, C, and D were increased significantly different (P<0,05) than control (treatment A). Supplementation of Saccharomyces spp.S-7 isolate culture in diets were increased significantly different (P<0,05) on dry matter (DM), organic matter (OM), crude protein (CP), and crude fibre (CF) digestability than control (unsupplemented). On the other hand were decreased significantly different (P<0,05) on abdominal fat, blood serum cholesterol contents, and concentration of N-NH3 on excreta of broiler. It was concluded that supplementation of Saccharomyces spp.S-7 culture (isolated from manure of Bali cattle samples), were increased live weight gains, carcass percentages, breast meat, and feed efficiencies of of broiler up to six weeks old. On the other hand were decreased abdominal fat, blood serum cholesterol contents, and concentration N-NH3 of broiler excreta. Keywords: Saccharomyces spp., probiotics, crude fiber, digestibility, ammonia, broiler