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Journal article
Selection of Khamir Saccharomyces s pp. Isolated from Colon of Native Chickens as a Probiotics Properties and has CMC-ase Activity
I Gst. Nym. Gde Bidura Desak Putu Mas Ari Candrawati Dewi Ayu Warmadewi
Volume : 32 Nomor : 2 Published : 2015, October
journal of biological and chemical research(JBCR)
Abstrak
The purpose of this research is to get isolates of Saccharomyces spp. as potentially as a probiotic agents and degrading of crude fiber (CMC-ase activity) were isolated from the colon of native chickens can be used in order to alleviate the negative effect of rice bran as poultry feed. The ability test of isolates has CMC-ase activity assay views of the clear zone surface area caused by isolates the user's media CMC (carboxymethyl celulose). The results of experiment showed that six isolates of Saccharomyces spp. (Gb5; Gb6; Gb7; Gb9, Gb10, dan Gb11) were isolated from colon of native chickens samples. The whole isolates of Saccharomyces sp. showed resistant grew on both in different temperature (100-450C), acid conditions (1.5-6.0), bile salts (0.20 to 0.60 NaDC) and has CMC-ase activity. All of isolates were potensial as probiotics sources and has a CMC-ase activity. But only two isolates of Saccharomyces sp. showed good potensial as probiotics sources and has CMCase activity (i.e. Saccharomyces sp.Gb7 and Gb9 isolates). The study showed that fermentation of rice bran used of Saccharomyces sp.Gb7 and Gb9 isolates culture could improve significant differences (P<0.05) on digestibility of its dry matter (DM), organic matter (OM), crude protein (CP), crude fibre (CF), and increased its metabolizable energy of rice bran. It was concluded that nutrient digestibility of rice bran might be improved by fermentation using both of Saccharomyces spp.Gb7 and Gb9 isolated from colon native chickens. The isolates (Saccharomyces spp.Gb7 and Gb9) can be used as a probiotic agency and crude fibre degradaded (has a CMC-ase activity) of feed. Key words: Probiotics, CMC-ase, Fiber and Rice bran.