ISOLATION OF CELLULOLYTIC BACTERIA FROM RUMEN LIQUIT OF BUFFALO BOTH AS A PROBIOTICS PROPERTIES AND HAS CMC-ASE ACTIVITY TO IMPROVE NUTRIENT QUALITY OF SOYBEAN DISTILLERY BY-PRODUCT AS FEED

This experiment was carried out to study implementation of Cellulolytic bacteria culture (isolation from rumen liquit of buffalo samples) as a probiotics agent can be used in order to alleviate the negative effect of soybean distillery by-product as feed. Eighteen of male Bali duckling was assigned to three treatments in a completely randomized design. Each treatment has six replications with one bird per replication (individual cage). The treatments were (i) unfermented soybean distillery by-product as control; (ii) fermented soybean distillery by-product by 0.30% Cellulolytics bacteria culture; and (iii) 0.60% Cellulolytics bacteria culture, respectively. The report on the first experiment showed that five isolates of cellulolytic bacteria (B-3, B-6, B-7, B-10, and S-13) were isolated from isolation from rumen liquit of buffalo samples in the first experiment. The whole isolates of cellulolytic bacteria showed resistant grew on both in different temperature (200-550C), acid conditions (3.0-6.0), and bile salt (0.20-0.60 NaDC). Isolates cellulolytics bacteria B-6 was potensial as probiotics sources and has CMC-ase activity. The study showed that fermentation of soybean distillery by-product using of isolates Cellulolytics bacteria B-6 isolate culture could improve significant differences (P<0.05) on digestibility of its dry matter (DM), organic matter (OM), crude protein (CP), crude fibre (CF), and increased its metabolizable energy of soybean distillery by-product. The content of dry matter, organic matter, and gross energy of soybean distillery by-product were no effected significantly different (P>0.05) by fermentation. On the other hand, fermentation caused increasing crude protein (CP) of the soybean ditellery by-product. It was concluded that fermentation of soybean distillery by-product by Cellulolytics bacteria B-6 isolate culture (isolation from rumen liquit of buffalo samples) could increase nutrient composition and digestibility of soybean distillery by-product as feed. Key words: Cellulolytic bacteria, soybean distillery by-product, crude fiber, digestibility