Degradation of Rice Straw Lignocellulosic Compounds by Lignocellulolytic Bacteria Inoculant from Bali Cattle Rumen Fluid and Termites

Background. Optimizing the use of rice straw which is the main waste of food crops as feed, is a vital step in developing an integrated livestock-farming business with the “SIMANTRI” pattern. The high lignocellulosic is the main limiting factor for use of rice straw as feed. Utilization of lignocellulolytic bacteria as starter inoculants for rice straw is a strategy developed in this study. Material and Method. Lignocellulolytic bacteria isolated from the rumen fluid of Bali cattle (R) and termites (T) use in this study, namely; R1=Pseudomonas aeruginosa strain BR9LS, R2=Bacillus subtilis strain BR4LG, R3=Bacillus subtilis strain BR2CL, R4=Paenibacillus dendritiformis strain BR3XY, and T1=Aneurinibacillus sp. strain BT4LS, T2=Aneurinibacillus sp. strain BT5LG, T3=Bacillus sp. strains BT3CL and T4=Bacillus sp. strain BT8XY. Eleven (11) inoculants were produced in this study were 9 inoculants of lignocellulolytic bacteria (BR1234; BT1234; BR12T34; BR13T24; BR14T23; BR23T14; BR24T13; BR34T12; and BR1234T1234), and 2 inoculants control (inoculants without lignocellulolytic bacteria/BR0T0 and inoculants of 10% bali cattle rumen fluid/BRL). Result.The study showed that the use of superior lignocellulolytic bacteria inoculants from rumen fluid of Bali cattle and termites and inoculant of bali cattle rumen fluid (BRL) were able to significantly increase the degradation of lignocellulosic compounds in rice straw as indicated by a decrease in the content of NDF, ADF, ADL, cellulose, hemicellulose, insoluble lignin, and silica, had increased of crude protein content, metabolite products and dry matter and organic matter in vitro digestibility compared than RSBR0T0. Conclusion.The inoculant formula BR23T14 is the best inoculant for produces the best quality fermented rice straw