DEGRADATION OF LIGNOCELLULOSIC SUBSTRATES BY BACTERIA CONSORTIUM OF BALI CATTLE COLON AND ORGANIC WASTE

A research has been carried out to evaluating potency of bacteria consortium inoculant formulated by elected superior bacteria isolated of bali cattle colon and organic waste as lignocellulosic substrates degrader. Evaluated based on degradation of lignocellulose substrates measurement using diameter of clear/diffusion zone and lignocellulase enzyme activity such as endoglucanase, exoglucanase. xylanase and lignase. This study showed that inoculant of bacteria consortium produce clear zone diameter 0.30 ? 0.72 cm, 0.27 ? 0.62 cm, 0.43 ? 0.78 cm, 0.21 ? 0.48, 0.29 ? 0.71 and 0.41 ? 0.80 cm respectively for carboxy methyle cellulose/CMC, avicel micro crystalin, xylan, tannic acid, rice straw, and rice brand substrates, while inoculant BW12C12 produced highest clear zone diameter on all lignocellulose substrates evaluated. Meanwhile for enzym activity, bacteria consortium inocullant coded BW12C12 has highest endoglucanase, exoglucanase, xylanase, and lignase enzyme activity respectively for carboxy methyle cellulose/CMC, avicel micro crystalin, xylan and tannic acid substrates. It was concluded that bacteria consortium inoculant coded BW12C12 was highest potential as lignocellulosic substrates degrader.