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IN
Journal article
Molecular characterization of Extended-spectrum beta-lactamases-producing Klebsiella pneumoniae isolated from clinical specimens at a tertiary-referral hospital in Denpasar, Bali, Indonesia
NI NENGAH DWI FATMAWATI Ni Made Adi Tarini NI NYOMAN SRI BUDAYANTI Putu Yuliandari
Volume : 21 Nomor : 2 Published : 2015, February
Advance Science Letters
Abstrak
Almost no study has been performed in Bali to characterize genotype of ESBL-producing Klebsiella pneumoniae. The aim of this study was to determine genotype of ESBL produced by K. pneumoniae isolated from clinical specimens in Sanglah General Hospital, Bali, Indonesia. Ninety-seven non-duplicative ESBL-producing K. pneumonia isolates were examined for 16s rRNA, and then with Duplex PCR to detect blaSHV and blaCTX-M genes, and with Uniplex PCR for blaTEM gene. Half of the isolates (50.5%) had coexistences of blaTEM, blaSHV and blaCTX-M genes. Presence of two genes, blaTEM and blaSHV; blaTEM and blaCTX-M; and blaSHV and blaCTX-M, were 12.4%; 15.5%; and 8.2%, respectively. This study showed the widespread of TEM-, SHV-, and CTX-M-type ESBLs in Bali. Prudent use of antibiotics can save our planet from multidrug resistance microorganisms (MDROs) threat.