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IN
Journal article
Molecular Characterization of Extended-Spectrum Beta Lactamases Producing Klebsiella pneumoniae Isolated from Clinical Specimens at a Tertiary- Referral Hospital in Denpasar, Bali, Indonesia
NI NENGAH DWI FATMAWATI Ni Made Adi Tarini NI NYOMAN SRI BUDAYANTI Putu Yuliandari
Volume : 21 Nomor : 2 Published : 2015, February
Advance Science Letters
Abstrak
Almost no study has been performed in Bali to characterize genotype of ESBL-producing Klebsiellapneumo- niae. The aim of this study was to determine genotype of ESBL produced by K.pneumoniae isolated from clinical specimens in Sanglah General Hospital, Bali, Indonesia. Ninety-seven non-duplicative ESBL-producing K.pneumonia isolates were examined for 16s rRNA, and then with Duplex PCR to detect blaSHV and blaCTX?M genes, and with Uniplex PCR for blaTEM gene. Half of the isolates (50.5%) had coexistences of blaTEM, blaSHV and blaCTX?M genes. Presence of two genes, blaTEM and blaSHV; blaTEM and blaCTX?M; and blaSHV and blaCTX?M???? were 12.4%; 15.5%; and 8.2%, respectively. This study showed the widespread of TEM-, SHV-, and CTX-M- type ESBLs in Bali. Prudent use of antibiotics can save our planet from multidrug resistance microorganisms (MDROs) threat. Keywords: Extended Spectrum Beta-Lactamase, Klebsiellapneumoniae, PCR, Sanglah General Hospital Denpasar.