The Influence of Euphorbia Milii Flower Extract in the Activity of Th17 through IL-17 Secretion in Mycobacterium tuberculosis Infected Mice

Abstract: The present study was conducted to study Euphorbiamilii flowerextract (EM) in increasingthe activity ofTh17 cells through IL-17 secretion. This was an experimental study with Post Test Only Control Group design. 24 BalbC, male,were divided in to 6 groups of treatment which were observed at first week. Group K1 and K2 are negative controls; P1 and P3 were given OAT and M.tb infected; while the P2 and P4 were infected M.tb, given OAT and EM. Terminations of K1, P1 and P2 were in the first week, while K2, P3 and P4 were terminated in the third week. Pulmonary organs were removed for examination of IL-17 by ELISA. The result showed mean of level of IL-17 in K1, P1, P2, K2, P3 and P4 respectively is 81.18; 90.00; 88.65; 87.53; 75.45 and 87.53 pg/ml. EM extracts increased the concentration ofIL-17 in the third weekhigher than thegroup thatdid not receivethe extractbut the difference wasnot significantly. It happened probably because a lot of factors that affect the stability ofIL-17, thus affecting the outcome. It is necessary for further research on the mRNA expression of IL-17, so that the influence factors can be minimized. Keywords: Euphorbia milii, Mycobacterium tuberculosis, Th17, IL-17