The Influence of Euphorbia Milii Flower Extract in the Activity of Th17 through IL-17 Secretion in Mycobacterium tuberculosis Infected Mice

Abstract: The present study was conducted to study Euphorbiamilii flowerextract (EM) in increasingthe activity ofTh17 cellsthroughIL-17 secretion. Thiswas an experimental study with Post TestOnly ControlGroupdesign. 24 BalbC, male,were divided in to 6 groups of treatment whichwere observedat firstandthird week. GroupK1and K2arenegativecontrols; P1andP3weregivenOAT and M.tbinfected; while theP2andP4were infected M.tb, givenOATandEM. Terminations of K1, P1andP2were inthe firstweek, while K2, P3andP4 were terminated inthe thirdweek. Pulmonaryorgans were removed for examinationofIL-17 by ELISA. The result showed mean of level ofIL-17 inK1, P1, P2, K2, P3andP4respectivelyis81.18; 90.00; 88.65; 87.53; 75.45and87.53 pg/ml. EMextractsincreased the concentration ofIL-17 in the third weekhigher than thegroup thatdid not receivethe extractbut the difference wasnot significantly. It happenedprobablybecausea lot offactors thataffect the stability ofIL-17, thus affecting theoutcome. It is necessary forfurther research onthe mRNAexpression ofIL-17, so thatthe influence factorscan be minimized. Keywords: Euphorbia milii, Mycobacterium tuberculosis, Th17, IL-17