PENGARUH PROPOLIS TERHADAP SEKRESI INTERLEUKIN-12 PADA SUPERNATAN KULTUR M AKROFAG DARI PENDERITA TUBERKULOSIS PARU YANG DIINFEKSI Mycobacterium tuberculosis

The uses of adj uvan i munot her apy has at t r act t he at t ent i on t uber cul osi s t r eat ment , because of t he i ncr easi ng pat i ent per sent age i n ant i t uber cul osi s agent . Pr opol i s i s bee gl ue, cont ai n of caf f ei c aci d phenet hyl est er ( CAPE) t hat assume i mmunomodul at or act i vi t i es. The ai m of t hi s st udy wer e t o assess t he r ol e of pr opol i s t o i nt er l euki n 12 secr et i on i n macr ophages cul t ur e f r om pul monar y t uber cul osi s pat i ent t hat i nf ect ed wi t h Mycobact er i um t uber cul osi s ( M t b) . Thi s st udy was t r ue exper i ment al usi ng post t est onl y cont r ol gr oup desi gn, wi t h sampel f r om per i pher al bl ood mononucl ear cel l s ( PBMC) of l ung t uber cul osi s pat i ent t hat di vi ded i nt o 3 gr oups wi t hi n 6 r epl i cat i on. Gr oup I as cont r ol gr oup wi t hout M t b i nf ect i on and pr opol i s i nt er vent i on; gr oup I I t r eat ment gr oup wi t h M t b i nf ect i on onl y; and gr oup I I I t r eat ment wi t h M t b i nf ect i on and pr opol i s i nt er vent i on. I nt er l euki n 12 secr et i on wer e measur ed 48 hour s post M. t b i nf ect i on. Resul t showed t hat means of i nt er l euki n 12 secr et i on at t hese gr oups ar e 11 pg/ ml ; 23. 33 pg/ ml ; 37 pg/ ml i n whi ch i nt er vent i on of pr opol i s 10 mg/ ml bef or e M t b i nf ect i on coul d i ncr ease i nt er l euki n 12 consent r at i on si gni f i canl y ( p< 0. 05) compar ed wi t h cont r ol gr oup and gr oup wi t h i nf ect i on of M t b onl y. We concl ude t hat pr opol i s coul d i ncr eases macr ophages act i vi t i es wi t h i n i ncr ease secr et i on of i nt er l euki n 12. Keywor ds: i nt er l euki n 12 secr et i on, pr opol i s, macr ophages cul t ur e f r om l ung t uber cul osi s pat i en