HIGH 8-OHdG COMPOUNDS CONCENTRATION AND LYMPHOVASCULAR INVASION ARE RISK FACTOR FOR INCREASES INVASIVE BREAST CANCER BEHAVIOR

Background: Free radicals produced by oxidative stress has long been associatedas one of the triggering factor of DNA mutation that lead to oncogenesis, especially in breast cancer. Serum level of 8-hydroxy2-deoxyguanisine (8-OHdG) is one of the most studied oxidative stress biomarker in nuclear and mithocondrial DNA damage. The objective of this study was to analyse the risk of high serum level of 8-OHdG ininvasive behaviour of breast cancer based on lymphovascular invasion (LVI). Method: this was a case-control study involving 66 breast cancer patients devided into 33 patients with positive LVI (LVI+) as case group and 33 patients with negative LVI (LVI-) as control group. This study was conducted in Laboratory of Biochemistry, Faculty of Medicine, Udayana University and Sanglah General Hospital Denpasar, Bali. Serum level of 8-OHdG was measured by ELISA. Results and Discussion: Increase of 8-OHdGserum level were associated with invasive characteristics in breast cancer patients (565.326 ± 282.655 ng/mL in case group compared with 326.336 ± 144,53 ng/mL in control group; p <0,05). Risk analysis has shown higher level of serum 8-OHdG were associated with increase risk of invasive behaviour of the cancer itself (OR: 13,913; 95% CI: 1,163 – 116,412). ROC curve analysis showed Area under the Curve (AUC) was 0.764 which indicates moderate predictive value. This result emphasized the role of oxidative stress in DNA damage and oncogenesis. Conclusion: high concentrations of 8-OHdG and lymphovascular invasion are positively associated with increased invasive breast cancer behavior.