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IN
Journal article
Evaluatian of Zoonotic Potency of Escherichia coli O157 H7 through Arbitrarily Primed PCR Methods
I Wayan Suardana Drh. Dyah Ayu Widiasih, Ph.D I GUSTI NGURAH KADE MAHARDIKA KOMANG JANUARTHA PUTRA PINATIH Dr. Budi Setiadi daryono
Volume : 5 Nomor : 11 Published : 2015, August
Asian Pacific Journal of Tropical Biomedicine
Abstrak
ABSTRACT Objective: To evaluate the zoonotic potency of Escherichia coli O157:H7 through arbitrarily primed-PCR (AP-PCR) methods as one of the DNA fingerprinting methods. Methods: A total of 14 isolates consisted of 11 isolates originated from human feces with renal failure symptoms, 2 isolates originated from cattle feces, and 1 control isolate were used in this study. DNA of each isolate was extracted, and their profiles were studied by using AP-PCR method with M13 F and M13 R arbitrary primers. Results: The results founded that all of 14 isolates had similarity range from 54.6% to 88.5%. Isolates KL-106(3) and KL-55(6) originated from humans showed the degree of similarity with isolates SM-25(1) and SM-7(1) originated from cattle as high as 85% and 77%, respectively. Conclusions: The high degree of similarity between isolates originated from cattle and human indicated the high potency of zoonoses. The results also concluded AP-PCR method as a briefly fingerprinting method in order to trace the epidemiological of E. coli O157:H7.