Antibiotic Resistance Profiles of Escherichia coli O157 H7 in Cattle at South Kuta, Badung Regency, Bali, Indonesia

Abstract: Escherichia coli O157:H7 is one of bacterium that known as a zoonotic agent. The spread of bacterium is usually through food contaminations and cattles are known as the main resevoir of this agent. The infections of E. coli O157:H7 can cause mild diarrhea to bloody diarrhea in young cattle and in adult are usually as a carriers. The humans infections by this agent can cause hemorragic colitis and hemolytic uremic syndrome (HUS). This study used five local isolates of E. coli O157:H7 i.e. FS.KS-5, FS.KS-17, FS.KS-36, FS.KS-44 and FS.KS-55 resulted from 60 faecal samples. The study was conducted by cultivating of isolate in sorbitol macConkey agar (SMAC), followed by testing on O157 latex aglutination and finally by testing on H7 antiserum. The sensitivity test of isolate againts various antibiotics was conducted using Kirby-Bauer methode. The isolates were tested on penicillin G, ampicillin, sulfamethoxazole and steptomycin. The results of study indicated all of isolates were resistant to penicillin G (100%). Moreover, sensitivity test to the ampicillin showed 80% resistant and 20% sensitive. Sensitivity test to the sulfamethoxazole showed 20% resistant, 40% intermediate and 40 % sensitive, as well as to the antibiotic streptomycin were 20% resistant, 20% intermediate and 60% sensitive. The results also showed three out of five isolates of E. coli O157:H7 (60%) namely FS.KS-17, FS.KS-44 and FS.KS-55 were resistant against two types of antibiotics (penicillin G and ampicillin). On the other hand, FS.KS-5 has shown resistant to four antibiotics. Therefore, it is necessary more studies in the future in order to track the evolution of this type of resistance among isolates.