C-1562T POLYMORPHISM OF MATRIX METALLOPROTEINASE-9 (MMP-9) GENE ASSOCIATED WITH ELEVATED LEVEL OF PLASMA MMP-9 CONCENTRATION IN PATIENT WITH ACUTE MYOCARDIAL INFRACTION (AMI) IN DENPASAR, BALI

Background:Acute Myocardial Infarction (AMI) is emergency medical condition which still has high mortality and morbidity. In recent decades, it prevalence has a tendency to increase, parallel with other chronic diseases. One of suspected contributing factor in AMI is the presence of C-1562T polymorphism on MMP-9 gene which increase plasma MMP-9 concentration and destabilize the plaque. However, this notion needs further confirmatory studies, as there are several contradictive reports regarding their association. This Study aimed todetermine the relationship between C-1562T polymorphism with the increase of MMP-9 concentration in AMI. Method: A cross-sectional was conducted in Cardiovascular Centre of Sanglah General Hospital. The peripheral blood samples were obtained from subjects and the DNA and blood plasma was isolated. The polymorphism was detected using PCR and RFLP while MMP-9 concentration was determined by ELISA. Results: The average concentration of plasma MMP-9 was found to be at 8.33 ng/mL which ranged from 0.74 ng/mL until 31.93ng/mL. The proportion of MMP-9 C-1562T polymorphism was 20% from all subjects. The average concentration of MMP-9 was higher than cut-off standard which is 0.6 ng/mL. Analysis within the group revealed that CT-genotype had significantly higher average MMP-9 compared with CC phenotype (11.27 ng/mL vs 7.65 ng/mL). Conclusion: C-1562T polymorphism appears to be significantly associated with AMI by increasing the concentration of MMP-9 in blood plasma.