Inhibition of Spermatogonia Formation on Little Wistar Rats

Testes are male gonads which play an important role in reproductive function. These organs are composed of germ cells, Sertoli cells, and Leydig cells that have been formed since the fetus. Each of these cells has different main function. However each cell synergize with one another. The formation and function can be disturbed by endocrine disrupting chemicals. The objective of this research was to reveal inhibition mechanism of spermatogonia formation of little rats by increasing dam rats estradiol level, inhibiting the formation of Sertoli cells, Leydig cells, and Androgen Receptors after the dam rats given the ethanol extract of tempeh during periconception period. The research design used was Randomized Post-test Only Control Group Design. The research was carried out at the Udayana University’s Biomedical Integrated Laboratory for 8 weeks, using 32 Wistar dam rats aged 12-13 weeks, and divided into 2 groups. The control was given aquadest; meanwhile the treatment was given ethanol extract of tempeh in 1 g / kg BW / day, contained genistein of 1.04 mg. The treatment was given 2 weeks before mating, during pregnancy, and breastfeeding. The observed data, including estradiol level of dam rats, number of Leydig Cells, Sertoli Cells, androgen receptors, and spermatogonia of little rats. The results showed that the average of Control vs Treatment Group for estradiol levels of dam rats are (49.17 ± 19.83 vs 181.19 ± 58.62), Sertoli cells are (67.69 ± 3.72 vs. 27.19 ± 7.89), Leydig Cells are (69.56 ± 3.12 vs. 31.50 ± 7.60), androgen receptors are (67.06 ± 4.54 vs. 27.75 ± 7.94), and spermatogonia are (314.25 ± 16.58 vs 234.06 ± 29.97). The data showed significant difference (p <0,05) of all variables between Control and Treatment Group. The number of Sertoli cells has the greatest effect on the number of spermatogonia (1.780). Conclusions: the ethanol extract of Wilis soybean tempeh in 1 g / kg BW/ day, given to the Wistar dam rats during periconception period was able to inhibit the formation of spermatogonia, directly or indirectly, by increasing estradiol level of dam rats, and inhibited the formation of Leydig cells, Sertoli cells, and androgen receptors.