CHANGES IN MALE REPRODUCTIVE SYSTEM INDUCED BY TEMPEH ETHANOL EXTRACT ADMINISTERED TO RATS FROM PRENATAL UNTIL WEANING PERIOD OF LIFE

Objective: Sertoli cell and spermatogonia are formed in the prenatal period, and alterations information process could affect the reproduction function in the adolescence and adult period. This study aimed to unveil the effect of tempeh’s isoflavone that was given from prenatal to weaning during childhood period toward the amount of Sertoli cell and spermatogonia. Method: The study design was randomized post-test only control group design with 15 female Wistar experimental mouse in the aged of 12 th – 13 th weeks was used and divided into three groups, namely, control groups (C) that were given aquadest, intervention Group 1 (I1) and intervention Group 2 (I2) that was given tempeh 0.5 g/kg body weight/day ethanol extract that contains 0.52 mg genistein and 5 g/kg body weight/day that contains 5.2 mg genistein, respectively. The intervention was given for a week before copulation, during pregnancy and lactation period. The observed parameters were Sertoli cell and spermatogonia of an early-born mouse. Result: The amount of Sertoli cell and spermatogonia were different between the three groups (p<0.05). The I2 group has the lowest amount of Sertoli cell and spermatogonia and shows a significant difference from the control group as well as I1 (p<0.05). Tempeh ethanol extract has a direct impact on reducing the amount of Sertoli cell to about 11% lower than control. It also affected the number of spermatogonia indirectly through its effect toward Sertoli cells. Conclusion: Intervention of tempeh ethanol extract to female Wistar experimental mouse from prenatal period to weaning child period could disturb the formation of Sertoli cell and spermatogonia of the early-born mouse.